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EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung <t>tissue</t> <t>IL-5</t> levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.
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EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung <t>tissue</t> <t>IL-5</t> levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.
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EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung <t>tissue</t> <t>IL-5</t> levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.
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EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung <t>tissue</t> <t>IL-5</t> levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.
Il 33 Quantikine Enzyme Linked Immunosorbent Assay Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems rat elisa kits
ZQG attenuates PMA-induced NETosis and inflammatory responses in human neutrophils via targeting the Nox4/ROS pathway. (A) ZQG suppresses oxidative burst and NET formation. Representative immunofluorescence images showing intracellular ROS (stained by DHE, red) and NETosis (marked by CitH3, green). Nuclei are counterstained with DAPI (blue). Scale bars: 20 μm. (B) Quantitative analysis of intracellular ROS levels. (C) Quantification of NETosis, expressed as the percentage of Sytox Green + cells. (D) Western blot analysis demonstrating the effects of ZQG and si-Nox4 on the protein expression of Nox4, p-Akt, and PADI4. (E) Densitometric quantification of Western blot results. (F) qPCR analysis of Nox4, PI3K, Akt, and PADI4 mRNA expression. (G) <t>ELISA</t> analysis of pro-inflammatory cytokine levels (IL-2, IL-5, IL-6, <t>IL-12,</t> <t>TNF-α)</t> in cell culture supernatants. Data are presented as mean ± SEM (n=10 independent experiments). ***p < 0.001 vs. PMA group.
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Image Search Results


EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung tissue IL-5 levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.

Journal: Current Issues in Molecular Biology

Article Title: Eupatorium lindleyanum DC. Suppresses Cytokine Storm by Inhibiting NF-κB and PI3K–Akt Signaling in Sepsis-Associated and Virus-Related Acute Lung Injury

doi: 10.3390/cimb48030333

Figure Lengend Snippet: EL inhibits NF-κB activation and attenuates the cytokine storm in SALI rats. ( A ) Serum IL-1β levels ( n = 6). ( B ) Serum GM-CSF levels ( n = 6). ( C ) Serum IFN-γ levels ( n = 6). ( D ) Serum TNF-α levels ( n = 6). ( E ) Serum IL-6 levels ( n = 6). ( F ) Lung tissue IL-1β levels ( n = 6). ( G ) Lung tissue IL-5 levels ( n = 6). ( H ) Lung tissue IL-6 levels ( n = 6). ( I ) Lung tissue IL-18 levels ( n = 6). ( J ) Lung tissue GM-CSF levels ( n = 6). ( K ) Lung tissue IFN-γ levels ( n = 6). ( L ) Lung tissue TNF-α levels ( n = 6). ( M ) Western blot analysis of NF-κB pathway proteins ( n = 3). ( N ) Relative densitometric ratio of p-IκBα/IκBα ( n = 3). ( O ) Relative densitometric ratio of p-P65/P65 ( n = 3). ## p < 0.01 compared to Sham. * p < 0.05 and ** p < 0.01 compared to MG.

Article Snippet: Kits for GM-CSF (E-EL-R0008), IFN-γ (E-EL-R0009), IL-5 (E-EL-R0558), and IL-18 (E-EL-R0567) were supplied by Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Activation Assay, Western Blot

ZQG attenuates PMA-induced NETosis and inflammatory responses in human neutrophils via targeting the Nox4/ROS pathway. (A) ZQG suppresses oxidative burst and NET formation. Representative immunofluorescence images showing intracellular ROS (stained by DHE, red) and NETosis (marked by CitH3, green). Nuclei are counterstained with DAPI (blue). Scale bars: 20 μm. (B) Quantitative analysis of intracellular ROS levels. (C) Quantification of NETosis, expressed as the percentage of Sytox Green + cells. (D) Western blot analysis demonstrating the effects of ZQG and si-Nox4 on the protein expression of Nox4, p-Akt, and PADI4. (E) Densitometric quantification of Western blot results. (F) qPCR analysis of Nox4, PI3K, Akt, and PADI4 mRNA expression. (G) ELISA analysis of pro-inflammatory cytokine levels (IL-2, IL-5, IL-6, IL-12, TNF-α) in cell culture supernatants. Data are presented as mean ± SEM (n=10 independent experiments). ***p < 0.001 vs. PMA group.

Journal: Frontiers in Immunology

Article Title: Pathological neutrophil extracellular traps hinder postoperative anal fistula wound healing and are attenuated by Zuoqing granule via suppression of the Nox4 pathway

doi: 10.3389/fimmu.2025.1730184

Figure Lengend Snippet: ZQG attenuates PMA-induced NETosis and inflammatory responses in human neutrophils via targeting the Nox4/ROS pathway. (A) ZQG suppresses oxidative burst and NET formation. Representative immunofluorescence images showing intracellular ROS (stained by DHE, red) and NETosis (marked by CitH3, green). Nuclei are counterstained with DAPI (blue). Scale bars: 20 μm. (B) Quantitative analysis of intracellular ROS levels. (C) Quantification of NETosis, expressed as the percentage of Sytox Green + cells. (D) Western blot analysis demonstrating the effects of ZQG and si-Nox4 on the protein expression of Nox4, p-Akt, and PADI4. (E) Densitometric quantification of Western blot results. (F) qPCR analysis of Nox4, PI3K, Akt, and PADI4 mRNA expression. (G) ELISA analysis of pro-inflammatory cytokine levels (IL-2, IL-5, IL-6, IL-12, TNF-α) in cell culture supernatants. Data are presented as mean ± SEM (n=10 independent experiments). ***p < 0.001 vs. PMA group.

Article Snippet: The concentrations of interleukin-2 (IL-2), interleukin-5 (IL-5), interleukin-6 (IL-6), interleukin-12 (IL-12), and tumor necrosis factor-alpha (TNF-α) in the supernatants were quantified using specific commercial rat ELISA kits (R&D Systems, Catalog # R6000B) according to the manufacturers’ instructions.

Techniques: Immunofluorescence, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture